A multiplexed droplet digital PCR assay performs better than qPCR on inhibition prone samples.
نویسندگان
چکیده
We demonstrate the development of a multiplex droplet digital PCR assay for human cytomegalovirus (CMV), human adenovirus species F, and an internal plasmid control that may be useful for PCR inhibition-prone clinical samples. This assay performs better on inhibition-prone stool samples than a quantitative PCR assay for CMV and is the first published clinical virology droplet digital PCR assay to incorporate an internal control.
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ورودعنوان ژورنال:
- Diagnostic microbiology and infectious disease
دوره 80 4 شماره
صفحات -
تاریخ انتشار 2014